Contact experts in Small RNA to get answers
2,269 views 178 posts
Questions related to Small RNA
Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....
12 December 2016 6,427 3 View
Had anyone used NGS library prepraration kit"CleanTag™ Small RNA Library Prep Kit" to prepare ultra low imput RNA? How about the library quality of this kit?
12 December 2016 4,876 0 View
I am working on some small RNA-Seq data and trying to normalize them. From literature, I found that people are using some housekeeping small RNA as reference for the normalization in qPCR. Can...
11 November 2016 667 3 View
02 November 2016 4,289 3 View
In our daily lab life we extract nucleic acids from vertebrate tissues or cell lines. For a candidates project we now want to extract small RNAs from snails early embryos (Lymnaea stagnalis)....
25 October 2016 1,780 1 View
10 October 2016 2,679 1 View
Hi guys, My question is regarding the pipeline for identification of phasiRNAs in plants. There are many papers that have used an analysis method but were not very informative on how to execute...
18 August 2016 7,075 2 View
08 August 2016 9,921 2 View
I am trying to grow bacterial cells in human plasma to test for sRNA differential expression. But the company I bought plasma from said that the complement degrades almost immediately and that my...
12 July 2016 7,597 3 View
I want to detect small RNA, for which i need end labeled single stranded probes. what are the options for florescent labeling the probes for detection?
11 July 2016 9,117 1 View
Hello everyone, I´m using the Agilent's Small RNA chip for detection of microRNA, but I´m not sure of the indicated concentration by the bioanalyzer (18% of miRNA), because I didn't observe the...
10 July 2016 7,923 4 View
07 July 2016 8,005 5 View
Hey guys, Is it necessary to use the small RNA enrichment protocol to do miRNA study in this mirVana miRNA isolation kit? Cuz i found that using the total RNA extraction to perform real-time PCR...
07 July 2016 4,865 6 View
Hello.....I have used glycogen for RNA isolation (Trizol protocol) in purpose of next generation sequencing. It worked perfectly though it is difficult to see the small RNA pellet. Then, I tried...
07 July 2016 4,925 5 View
07 July 2016 279 3 View
07 July 2016 2,062 1 View
07 July 2016 9,255 2 View
I am detecting ~15 different miRNAs in serum samples. For normalization it seems most people use spike-in (cel-miR-39) or endogenous small RNA. I see various studies normalize to multiple...
06 June 2016 3,061 2 View
I'm looking for suggestions for papers to start from (or links to heated blog debates) in researching the evolution of thought over the last few decades regarding the causal role of histone...
06 June 2016 4,784 4 View
02 June 2016 7,743 3 View
Hi, I've seen it mentioned that modifications to the tri-reagent protocol (sigma) must be made in order to extract miRNA (included in the total RNA). Can anyone describe those modifications? In...
05 May 2016 3,369 3 View
Dear all, I'm currently preparing small RNA library for Illumina sequencing. I have two samples: one is wild-type cells, and the other is mutant cells in which one of the miRNA biogenesis factor...
29 April 2016 3,641 4 View
04 April 2016 1,441 5 View
Sequencing has done on Illumina Platform and Raw reads in FASTQ file format. How to check the read quality?
04 April 2016 2,375 5 View