Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

What a 50% positive predictive value mean?

I want to explain a threshold of 50% PPV to describe how some assays perform when the prevalence of disease is high or low

07 October 2020 8,657 6 View

Author who is not actually an author of some of my publications?

I came out to realize that a person who is enlisted as an author of some publications that I am the REAL AUTHOR, is marked instead of me. How do I change that? Example: Standardized sampling...

21 September 2020 378 7 View

How to increase the short circuit current density in a perovskite solar cell?

I'm optimizing the procedure for making inverted PSCs: ITO / PTAA(8nm) / CsMAFA(320nm) / PCBM(60nm) / BCP(9nm) / Ag(100nm). The cells look very good, I have a very good FF (0.70) and Voc (1.0V),...

16 September 2020 6,286 8 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

Sensors and Systems for IIoT?

13 March 2020 7,295 3 View

Does someone know the cause of stains appearing on n-i-p Perovskite Solar Sells?

I did the procedure described by saliba ( How to Make over 20% Efficient Perovskite Solar Cells in Reg... ) to make n-i-p PSCs. However, after perovskite deposition stains appeared in the cell...

29 October 2019 8,012 3 View

How to process small samples for histology?

Hi, I have very small samples of about 1x2mm (dental pulp tissue) and I need to process them for histology (dehydration, parafine inclusion, stainning with HE, etc). The problem is that the...

18 September 2019 1,180 4 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View