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Questions related to Rodent Models
I am studying the Brown adipose tissue Thermogenesis and would like to know the appropriate age of mice and its body weight to perform Cold exposure ( 4 degree ) experiment and also to study beta3...
08 August 2015 3,858 2 View
Rodent model of colon cancer. Is the ORAC test reliable or with any biological relevance to measure antioxidants capacities in plasma of mice ?
08 August 2015 6,767 3 View
How can I do intracerebral administration in suckling mice? I know in the adult mice, we can use 30G insulin needle, (8 mm), and sets of 5.96 mm PE10, so the syringe needle about 2.04 mm, it will...
08 August 2015 7,529 2 View
Ca we use female rats for chemical induced lung cancer.?
08 August 2015 4,233 14 View
We dissolved 1 mg/ml CNO in DMSO (2% of final volume). Our rats get daily injections (they are Brown Norway rats) and consistently develop lesions at the points of injection. Does anyone have any...
08 August 2015 8,834 5 View
I want to isolate connexin from rat brain in milligram quantity. I have been suggested to first isolate the crude membrane faction and then only connexin can be obtained. At present I am trying to...
16 July 2015 5,767 3 View
Even with concentration as high as 2000 uM?
07 July 2015 7,566 2 View
I have been having some rats die during post-op (prior to waking from i.m. ketamine/xylazine injection), and I am thinking the catheter length may have something to do with it...I did a post-op...
07 July 2015 1,361 3 View
07 July 2015 3,082 3 View
In the experiments of antihyperglycemic effects of natural products, many papers used metformin and other papers used sulfonylureas in STZ diabetic rats. I believe that sulfonylureas are...
07 July 2015 6,935 12 View
Hi everyone, I am working on gut cannulation to determine some specific proteins in gut. So I have to collect the gastric fluid as pure as possible for running western blot. But the problem is the...
07 July 2015 2,820 4 View
Hi, I'm trying to clear up some data obtained from the 5-CSRTT. Data showed some differences in both correct and reward latencies (time required to respond correctly after the onset of the...
07 July 2015 4,661 5 View
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07 July 2015 8,122 2 View
06 July 2015 6,351 3 View
We get fantastic AAVDj transduction of 293 cells (human). However in comparison we find that mouse N2a, NIH 3t3, and Ht22, and Rat2 (rat) cell lines do not transduce well at all. Thank you in...
18 June 2015 5,552 1 View
06 June 2015 9,504 0 View
1)There are many articles giving information that systemic administration of LPS increases pro inflammatory cytokines. 2)There are also articles giving information that systemic administration of...
06 June 2015 2,018 4 View
It' s my understanding that for TEM analysis, tissues must be fixed in glutaraldehyde (or 2% PFA and 2% GTA solution) to be embedded in resin, whereas tissues fixed in PFA is embedded in paraffin.
06 June 2015 3,884 3 View
I work in a lab interested in delirium and cognitive disorders related to Intensive Care Unit stay. We have plenty of biochemical data, but we would like to perform some behavioral tests in mice...
06 June 2015 3,417 2 View
In the case that a female rat delivered only one or two pups, what can I do? and how can I deal with it especially that it becomes so nervous? How can I deal with a female rat immediately after...
06 June 2015 7,235 4 View
We will make a sporadic Alzheimer's disease (SAD) model in mice. We will use streptozotocin icv way to make SAD.
06 June 2015 9,252 6 View
I am available with NIH outbred-multicolored guinea pigs and want to setup an experiment to measure the substance P modulation in diseased condition. On referring, the article works used Dunkin...
06 June 2015 1,448 1 View
Hello all, the only case I am aware is Arcis & Desor, 2003. Would greatly appreciate any additional leads.
06 June 2015 588 1 View
I'm now practising the internal carotid artery injection on mice, but everytime I found that the cells were all in the lung. Perhaps I injected the cells into the vein, but not the artery. Is...
05 May 2015 6,190 4 View