I want to isolate connexin from rat brain in milligram quantity. I have been suggested to first isolate the crude membrane faction and then only connexin can be obtained. At present I am trying to implement protocol proposed by Suski et al. 2014 for isolation of crude membrane fraction. But I don't have much idea for further purification of connexin.

Can someone suggest any other approach for isolation of connexin from rat brain ?

Thanks a lot.

http://www.nature.com/nprot/journal/v9/n2/full/nprot.2014.016.html

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