What are the methods for predicting DNA-binding proteins?

More Mahendra B Thapa's questions See All

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to improve the adhesion between germanium and silicon monoxide thin film layers using EBEAM evaporation?

I want to deposit multilayer coating of germanium and silicon monoxide on silicon wafer using ebeam evaporation?

21 February 2021 2,141 3 View

Can a compound have LogP value around 0.05 and LogD value around 4.02?

I understand that there might be huge differences between LogD and LogP for ionizable compounds. Normally, logD should be lower than logP for ionizable compounds. But can LogD value be...

05 January 2021 6,714 0 View

How to separate epithelial cells?

Hello everybody, Hope you all are doing fine during this difficult time. I am trying to culture mouse oviductal epithelial cells but, the problem is when I culture them there is a mixture of...

31 October 2020 2,640 4 View

How do an institution develop a linkage with the industries for better training facilitations to its students? What should be the modus-operandi?

What should be the recommended procedure for developing the linkage with the industries for imparting training/internship to the students; industries are reluctant to do so.

27 October 2020 2,576 16 View

What can you interpret from the estimate of NDVI and LST?

I had estimated the Land surface Temperature and Normalised Difference Vegetation Index, the result shows they have positive relation both increases in lower south part areas and higher in upper...

11 September 2020 6,021 4 View

Can entropy be reversed? In the Sci-Fi movie TENET, the concept is used to reverse time, is it possible theoretically?

Second Law of Thermodynamics states that as usable energy is lost, chaos increases - and that progression towards disorder can never be reversed.

01 September 2020 2,528 37 View

CG relaxation with B3LYP in VASP?

I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...

23 August 2020 8,848 4 View

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

What has been going on regarding blood transfusion and heart transplants despite Covid-19?

Heart transplant and blood transfusions are very sensitive whether or not there be any pandemics. How do you think is the pandemic affecting these and how could this be managed, like how blood...

28 May 2020 3,283 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Sam Tonddast-Navaei

Dear Mahendra,

If your sequences are for human proteins you can find the per-calculated results on our website (http://cssb.biology.gatech.edu/skolnick/webservice/DBD-Threader/index.html). If not, you can send your list of sequences to us and we will run them for you locally.

Hope that helps.

Sam