Can someone help with my problem with silver staining?

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Vello Tõugu

Chloride ions in the gel?

Susanna Kärkkäinen

Simple question but some don't think about it - do you use MilliQ water? And do you make the gel yourself or buy a commercially available gel?

Geetika Sirhindi

Once try to do staining in dark.

Adam B Shapiro

As Susanna Karkkainen and Vello Tougu suggested, it is likely that your gel is in a solution that is contaminated with salts, especially chloride. Silver chloride is insoluble in water.