We have thought about glass beads and sonication:
Is there a frequency one can use to sonicate and lyse open eukaryotic cells but leave the bacteria intact?
Alternatively, is it reasonable to think we can purchase a specific size of beads to beat the guts cells open, largely leaving the bacteria population alone?
Any other leads or ideas would be greatly appreciated! Thanks RGC.
0.25% Triton X-100 is commonly used in bacterial invasion assays to pop the mammalian cells and release the bacterial cells for enumeration by plate counts.
Beside detergent, you can use ball bearing homogenizer which we often use for phagosome purification and will lyse the eukaryotic cells not the bacterial population.
Thank you both for your replies and suggestions. I will look more into this.
We use 0.1% TX-100 in our lab to lyse mammalian cells to count internalized bacteria and it works well.
Hi Malachi,
Did Tx-100 work for you?. I wanted to do selective lysis of mosquito midgut cells without affecting associated microbiota
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