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Questions related to Protein Expression
What is the importance of Kozak sequence in protein expression during cloning?
20 July 2018 6,767 4 View
HI.... I am facing a weird problem.., I have retro-viral plasmid with selection marker conjugated to IRES; from which I produced virus and infected them to NIH-3T3 cells. After infection I could...
13 July 2018 467 4 View
I am a beginner studying MMP - 9. Expression of MMP-9 increases after injury stimulation such as LPS. Why does MMP-9 protein rise in as little as 6 hours? Is MMP-9 regulated by immediate early...
07 July 2018 9,404 0 View
07 July 2018 1,884 4 View
Does anybody have some positive experience with enhancing the protein expression in HEK 293 cells? I am working with concatamer of ion channel and performing whole-cell patch clamp measurements....
07 July 2018 5,540 9 View
I am currently using a lac-based vector for periplasmic expression of proteins in E. coli. I now want to re-design the vector and express the proteins under the control of the T7 promoter in a DE3...
07 July 2018 4,651 2 View
Presently I'm working on a host-vector combination, where the E. coli host is K-host and the vector is p15A ori-based vector with tetracycline as selection marker. The problem I'm facing is the...
07 July 2018 640 0 View
07 July 2018 481 5 View
I am doing survival analysis. separating the patients in groups of low and high exression of protein A gave no significant difference in survival analysis. However, by studying correlation...
07 July 2018 3,782 2 View
Can you describe briefly?
07 July 2018 2,813 0 View
Most protocols seem to just state 3 hours of growth.
07 July 2018 2,772 3 View
I am workig on Non LTR Retrotransposon and I want to find out from where should I insert my sequence? Just a breif background- The component I am working on is R9 which targets 28S rRNA . I need...
30 June 2018 6,466 3 View
hi all, i'm having a problem to express my gene in E.Coli (BL21) of a nucleation peptide. nowadays i'm suspecting that it might be because of a too short spacer in my vector: (the "map", and the...
28 June 2018 3,405 5 View
Currently trying to express protein in S.cerevisaie,peSc-His vector which has Gal promoter for induction.Did expression on enriched YPD/YPG media,suggested SD/SG media and addition of 1%galactose...
06 June 2018 3,328 0 View
I am doing ligation of my gene size 2.7kb in vector pet28a at 160C overnight. After that I transform my mixture in DH10B or DH10alpha. Most of the time i get no colonies and sometimes i get very...
06 June 2018 2,266 0 View
06 June 2018 5,352 4 View
Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...
06 June 2018 6,656 4 View
06 June 2018 5,373 6 View
I am using pET28a vector for protein expression. This vector is containing two type of origin of replication i.e pBR322 Ori and f1 Ori. The f1 is the bacteriophage origin of replication which...
28 May 2018 3,681 4 View
I mean, can ssDNA with promoter, RBS and ORF be stabilized and express protein in vivo?
05 May 2018 1,754 0 View
I want to ask why we always do a starter preculture step during protein expression ? Why don't we just start the main culture directly ?
05 May 2018 4,614 2 View
Hi, I am attempting protein expression using the PichiPink system. I've noticed that the cultures of untransformed cells are not always pink, even when the cells used to start those cultures...
05 May 2018 7,978 0 View
Hi, I am working on protein expression by Pichia pastoris. I focus on mixed feed to get higher result. I read many papers. Some of author said that glycerol is repression the AOX1 promoter, which...
05 May 2018 3,266 0 View
05 May 2018 2,779 5 View