Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers
is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is in this result ?
In my experience, mesenchymal stem cell basically express many lineage markers by default. These included as neuronal and chondrogenic markers. As for the cardiomyocytes, It doesn't expect that they do express such markers. You must be notified that cell adhesion of the MSCs may introduce linage differentiation of them, so higher passage (above 5th) or high confluencey must be avoided, precautionary. Myocyte differentiation is the most spontaneous differentiated lineage in the MSCs.
Article Cell-to-cell contact induces mesenchymal stem cell to differ...
MSCS can express the markers you referred to once they start to differentiate or are exposed to an environment that favours cardiomyocyte differentiation.
please refer to:
PLoS One. 2014 Apr 1;9(4):e93222. doi: 10.1371/journal.pone.0093222. eCollection 2014.
As mentioned by others something in your culture conditions is causing your MSCs to express cardiomyocyte markers. You can try changing the culture conditions and then assay for the markers
Hello
i agree with researchers . Check your culture conditions
Good Luck
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