No colonies after transforming the ligation mixture in DH10B cells?

06 June 2018 0 2K Report

I am doing ligation of my gene size 2.7kb in vector pet28a at 160C overnight. After that I transform my mixture in DH10B or DH10alpha. Most of the time i get no colonies and sometimes i get very small colonies. when i screen these colonies it shows no gene and vector. Please give suggestion to overcome this problem.

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