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Questions related to Protein Expression
Having not so much experience with cell-free expression systems, I do have a general question. I am using a commercial RTS 100 E.coli kit to express eGFP. When following the expression on a tecan...
26 January 2019 2,342 3 View
I am in a need of molecule which induces the protein expression of PPARalpha gene. Please suggest.
08 January 2019 7,521 2 View
kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculationon CYM liquid media in stress experiment. and I wanted to make sure that my inoculum is homogenous so i used a...
07 January 2019 8,828 0 View
01 January 2019 5,798 3 View
01 January 2019 8,336 2 View
Hello, I am working with a kind of mutated protein and it seems that at the transcriptional level there is no change in the gene expression compared to wild type where as in western blot I can...
01 January 2019 5,979 2 View
periplasmic expression or cytoplasmic expression in e.coli for production antibody fragments How to optimize periplasmic protein expression in E.coli (BL21) for antibody fragments with...
01 January 2019 5,418 5 View
Dear Scientists After protein expression, I did periplasmic and total protein extractions. I did the dot blot test to see if my proteins have been expressed. well, The dot blot result was perfect...
01 January 2019 6,358 7 View
hello At which frequencies values a codon is considered bad for protein expression in a given vector. best regards
12 December 2018 8,082 9 View
I am looking for a database which provides information on the change in the expression of protein before and after chemotherapy. Any information in this regard would be appreciated.
12 December 2018 4,410 7 View
I'd like to perform a comparative proteomic between two different breed lines of chicken to identify differential protein profile, protein expression but I have less experience in LC-MS/MS...
12 December 2018 826 0 View
I have been trying to get a sample of bacteriophage CE6 (which expresses T7 polyemerase) for protein expression studies. The lysogenic form of this is the (DE3) you see in genotypes of cells used...
15 November 2018 3,257 3 View
Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...
13 November 2018 3,980 4 View
11 November 2018 3,487 4 View
I have noticed several times that there are hard particles in the Pichia expression medium that block pipette tips when I try to wash the cells with buffer. I looked at these particles under the...
11 November 2018 5,720 4 View
Currently, we are expressing low molecular weight antibodies of about 15 kilodaltons in Escherichia coli, but by mutating only one amino acid, the expression is markedly lower compared to WT.Is...
11 November 2018 9,046 0 View
Based on my experimental results, what's the effectiveness of the control of protein expression in the pET system used.
11 November 2018 6,543 0 View
I have to co-culture human Mesenchymal stem cells (adherent) with leukemia cells (suspension) for a few days with treatments and then isolate leukemia cells and hMSCs separately fro mRNA and...
11 November 2018 9,083 2 View
I accidentally added double dose yeast extract in my LB broth for protein expression. I am wondering if it can be used.
11 November 2018 1,375 3 View
Hi, I am interested in recommendations of expression vectors for expression of proteins in E.coli and other Gram negative bacteria (potentially) that are independent of the T7 TNA polymerase; eg...
11 November 2018 3,889 0 View
So, I am reading a paper and I seem to be confused. In this paper they did a ChIP assay to observe the promoter region of p21 to determine if there was an interaction with p53. They claim their...
11 November 2018 5,188 2 View
09 November 2018 4,775 2 View
question as above
19 October 2018 320 2 View
Dear all, I would like to know whether addition of IPTG before (37 degC) and after lowering induction temperature (16 degC) affect protein expression levels? *add IPTG before = add IPTG when 37...
18 October 2018 9,106 6 View