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Questions related to Protein Expression
I was wondering if anyone has ever extracted proteins from insect larvae to determine protein expression and what the used protocol was. I would like to know what methods are out there and try to...
10 October 2018 9,848 0 View
Hello, for an experiment I'm considering doing, I would need to express a protein in a DE3 strain that would be able to use nitrate as final electron acceptor for respiration. I know that a few...
10 October 2018 5,538 1 View
I am currently trying to express human tyrosinase in E.coli and as you know this enzyme being a membrane protein it comes with a fair share of difficulties relating to expression in BL21(DE3)....
10 October 2018 7,811 3 View
question as above
10 October 2018 4,387 2 View
I am working with Caco-2 cells. I seed them on transwell filter. In our group TEER has been used to assess the the monolayer integrity. However TEER has been unreliable due to high flactuation. We...
10 October 2018 9,400 9 View
I am using BL-21 for chitinase protein expression please guide me how much concentration of IPTG and its volume per litter will be use for protein induction. Kindly share the protocol for inducing...
10 October 2018 5,380 9 View
09 October 2018 8,418 1 View
04 October 2018 7,121 3 View
Dose anyone know how to generate the 3D surface plot of IHC picture? I read a paper ( doi: 10.1002/cam4.231 ) mentioning the technique/software named "Protein Expression Quantifier" in quantifying...
09 September 2018 2,197 1 View
I have tried to overexpress and purify a membrane protein in HEK293T by PiggyBac system with PA-tag. However, the protein expression level is very weak, so it is difficult to purify this protein...
09 September 2018 700 3 View
tRNA level in E. coli BL21 strain is ~15% total. I wonder what is tRNA fold increase in strain Rosetta (DE3) since it carries pRARE plasmid.
15 August 2018 6,121 2 View
please contact me for details... Thanks
14 August 2018 2,925 2 View
I used to work with M9 minimal media way back for E coli protein expression, but I didn't recall the rationale. Couple years later I read several papers using M9 media for protein expression, I...
11 August 2018 1,958 4 View
My gene is cloned in pET28a+ and in then transformed into BL21 DE3. My gene size is about 70 kDa. I have used 1 % glucose during induction and using Terrific broth as a medium. Initially also i...
08 August 2018 2,499 9 View
08 August 2018 3,870 2 View
08 August 2018 1,743 2 View
08 August 2018 2,764 3 View
I need to express a recombinant plant protein in bacterial system. Kindly suggest me a Gateway expression vector with GST/ His/HA/myc tag.
08 August 2018 4,878 6 View
I am looking for a protein whose expression level does not change during the growth of S. aureus.
08 August 2018 1,149 3 View
I am interested in looking at protein profile after the treatment on the breast cancer cells, using Proteomics. However, I am not quite sure about the time point at which it will be the best to do...
08 August 2018 7,338 3 View
Hi, I have confused by this question. I have seen an yeast parental strain BY4741 on some website. Is the yeast parental strain BY4741 the same as strain BY4741? Can it be used for protein expression?
08 August 2018 4,581 0 View
What is the importance of Kozak sequence in protein expression during cloning?
20 July 2018 6,695 4 View
HI.... I am facing a weird problem.., I have retro-viral plasmid with selection marker conjugated to IRES; from which I produced virus and infected them to NIH-3T3 cells. After infection I could...
13 July 2018 432 4 View
I am a beginner studying MMP - 9. Expression of MMP-9 increases after injury stimulation such as LPS. Why does MMP-9 protein rise in as little as 6 hours? Is MMP-9 regulated by immediate early...
07 July 2018 9,369 0 View