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Questions related to Protein Expression
The method fo measuring GSH levels, GST, GPx and catalase activities in plasma please ?
04 May 2023 6,988 1 View
Hello, can anyone please describe to me an effective way to amplify a plasmid backbone. When I use my primers, I get a lot of non-specific priming and do not get a strong enough band (if any) at...
03 May 2023 1,470 4 View
Need to confirm the presence of protease before starting the extraction process
21 March 2023 2,279 1 View
I centrifuged the cells under 300g, 5 min. I can see a clear cell pellets. Then I discard supernatant and dissolve the pellets to count the cell number by trypan blue staining. But I only see few...
02 February 2023 1,516 2 View
We need to figure out how the Origin software processing the data from a Microcal VP ITC calorimeter computes the values of concentrations given in its output. They are not simply computed...
30 January 2023 7,221 4 View
I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
24 January 2023 5,128 3 View
due to colonies/agglomerates Streptomyces spp. forms in broth, I cannot use standard simple techniques such as OD600 or viable count to check isolates' development over time or survivability in...
22 December 2022 8,827 1 View
Hi, I am working with HEK293T cells. I have to make lysate for Co-IP using Triton X-100 Instead of 1% NP-40 which %age of Triton X-100 will be OK?
07 December 2022 938 0 View
Can anyone explain the calculation of GUS activity using fluorometric MUG assay. I could not find a detailed explanation of the calculation part. Thanks in advance.
30 November 2022 4,395 0 View
I want to check the role of one protein in neuronal cell line. For this, I'm going to use siRNA knock down of gene for that protein, in order to check whether knock down gene can effect on cells...
22 November 2022 3,869 2 View
Hi all, I want to express differents constructs of Ab such nanobodies or scfv in E. Coli and using pOPIN vectors ( vectors that we are using in our lab). I know there are some complications in...
21 November 2022 2,625 3 View
I am working on structure-activity relationship of oat protein derived peptides . The peptides sequence have poor water solubility, so i want to know which solvent is good to dissolve it, without...
01 November 2022 1,808 7 View
Hello. I am a Ph.D. student and for the first time, I am using a UV-vis spectrophotometer (ONDA UV-Vis 21). My question is about how to analyse the total amount of organic carbon compounds found...
10 October 2022 2,200 4 View
Hello, Would you please let me know, What is the best culture medium to increase Streptomyces pilosus production?
08 June 2022 8,690 4 View
After creating clusters from MCL algorithm via Clustermaker2 app in cytoscape how to analyse it and find biological relevance?
10 March 2022 4,566 3 View
My ques is is mercuric chloride make in hcl solution or in distill water¿ when we remove percentage and 2 ques is mercuric chloride unit is mg or ml or g.
28 February 2022 1,968 2 View
How to measure the diameter of plaque
22 February 2022 3,397 4 View
Dear community, I want to use the Friedman-test in combination with the Dunn-test on my data in GraphPad Prism. However, I have some missing values, so the software is not able to perform the...
24 January 2022 8,801 1 View
I have purified a recombinant protein and I am not getting any protein activity in activity assay. Protein contains histidine tag. Is there possibility that his tag is interfering with protein...
14 December 2021 8,137 4 View
Hi, I am working with pEGFP-N3 SV40 origin containing plasmid to assess transfection efficiency in different cell types. I am struggling to compare the efficiency of transfection between two cell...
04 July 2021 8,466 0 View
I use vcftools to calculate FST between different populations, based on resequencing data. But the values of FST are between 0.5 and 0.9. My research object is an endangered plant. I want to know...
21 June 2021 7,382 1 View
Hi there, I am trying to purify a recombinant His-tag protein from insect cell (baculovirus expression system) I used buffer containing %10 glycerol, 50mM SODIUM PHOSPHATE, 300mM NaCl , 20mM...
19 February 2021 6,897 6 View
i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions
16 February 2021 9,088 3 View
I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...
11 February 2021 4,001 3 View