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Questions related to Protein Expression
Hello. I've been trying to transfect plasmid DNA to my breast cancer cell lines (BT-474 and SK-BR-3) with Thermo Fisher's Lipofectamine 3000 reagent. According to the thermo fisher, those cells...
25 March 2024 9,500 5 View
WHAT IS THE ROLE OF NaOH IN THE REDUCTION?
18 January 2024 7,970 0 View
Hello. I have a question about these two behaviors, the Warburg and Crabtree effects. I am a master's student and my teacher said to me that Warburg occurs in cancer cells and Crabtree in yeast,...
18 January 2024 3,428 2 View
why some plant cultivars are susceptible and some are resistant to plant parasitic nematodes? which mechanisms can make plant resistance and what is its geneticsl base?
16 January 2024 3,047 2 View
I have expressed an Fc fusion protein in 293F cells, and the expression in the supernatant was so good, but when I purified it with Protein G agarose and Protein A agarose, it cannot be eluted. I...
06 November 2023 9,566 1 View
Hi everyone I have 2 vectors and I want to transform these into one bacteria cell (SHuffle strain). Do you think co-transformation (Transforming 2 vectors together in one step into the competent...
30 August 2023 4,081 4 View
Hello all, I have been trying to do immunoprecipitation and I am seeing big particles of magnetic beads in the cell lysate That doesn't seem to dissolve. Do y'all know the possible reason? I have...
01 August 2023 7,125 0 View
The method fo measuring GSH levels, GST, GPx and catalase activities in plasma please ?
04 May 2023 7,052 1 View
Hello, can anyone please describe to me an effective way to amplify a plasmid backbone. When I use my primers, I get a lot of non-specific priming and do not get a strong enough band (if any) at...
03 May 2023 1,525 4 View
Need to confirm the presence of protease before starting the extraction process
21 March 2023 2,348 1 View
I centrifuged the cells under 300g, 5 min. I can see a clear cell pellets. Then I discard supernatant and dissolve the pellets to count the cell number by trypan blue staining. But I only see few...
02 February 2023 1,567 2 View
We need to figure out how the Origin software processing the data from a Microcal VP ITC calorimeter computes the values of concentrations given in its output. They are not simply computed...
30 January 2023 7,276 4 View
Good evening I would like to know if anyone can provide me with methods for calculating two enzymes, Gluthation Transferase (GST) and Glutathione Reductase (GR) Thank you for your help
26 January 2023 5,090 2 View
I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
24 January 2023 5,212 3 View
due to colonies/agglomerates Streptomyces spp. forms in broth, I cannot use standard simple techniques such as OD600 or viable count to check isolates' development over time or survivability in...
22 December 2022 8,877 1 View
Hi, I am working with HEK293T cells. I have to make lysate for Co-IP using Triton X-100 Instead of 1% NP-40 which %age of Triton X-100 will be OK?
07 December 2022 988 0 View
Can anyone explain the calculation of GUS activity using fluorometric MUG assay. I could not find a detailed explanation of the calculation part. Thanks in advance.
30 November 2022 4,453 0 View
I want to check the role of one protein in neuronal cell line. For this, I'm going to use siRNA knock down of gene for that protein, in order to check whether knock down gene can effect on cells...
22 November 2022 3,937 2 View
Hi all, I want to express differents constructs of Ab such nanobodies or scfv in E. Coli and using pOPIN vectors ( vectors that we are using in our lab). I know there are some complications in...
21 November 2022 2,682 3 View
I am working on structure-activity relationship of oat protein derived peptides . The peptides sequence have poor water solubility, so i want to know which solvent is good to dissolve it, without...
01 November 2022 1,854 7 View
Hello. I am a Ph.D. student and for the first time, I am using a UV-vis spectrophotometer (ONDA UV-Vis 21). My question is about how to analyse the total amount of organic carbon compounds found...
10 October 2022 2,267 4 View
Hello, Would you please let me know, What is the best culture medium to increase Streptomyces pilosus production?
08 June 2022 8,749 4 View
After creating clusters from MCL algorithm via Clustermaker2 app in cytoscape how to analyse it and find biological relevance?
10 March 2022 4,629 3 View
My ques is is mercuric chloride make in hcl solution or in distill water¿ when we remove percentage and 2 ques is mercuric chloride unit is mg or ml or g.
28 February 2022 2,027 2 View