I have to co-culture human Mesenchymal stem cells (adherent) with leukemia cells (suspension) for a few days with treatments and then isolate leukemia cells and hMSCs separately fro mRNA and protein expression. Could anyone suggest different methods to do so. Thank you!
You could separate by magnetic CD45+ selection (stemcell technology or miltenyi). In addiiton, you could lightly wash and the majority of the cells collected are going to be lymphocytes, since MSCs are plastic adherent and lymphocytes proliferate in suspension; however they are not going to be 100% pure, unless you add a magnetic isolation step.
hi
do the MSc and leukaemia cells need to be in contact. If not you could put the leukaemia cells into 0.4µm transwell inserts for co-culture.
cheers
kay
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