Please suggest me an inducer of PPAR alpha protein expression?

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Can someone tell me estradiol inducible gene in brain endothelial cell lines?

I want to know a gene strongly altered by 17 beta estradiol in human brain endothelial cells, so that i can take that gene as a control for the system.

04 May 2017 2,442 3 View

Could anyone guide me on procedure to dissolve and make dilutions of 17 beta estradiol?

I am trying to perform gene expression experiment with 17 beta estradiol using HepG2 Cell lines. Somehow i am not able to get any induction or akteration. It looks like i am doing something wrong...

31 December 2016 7,292 5 View

For how many days i have to culture cells in phenol red free media before giving E2 treatment?

I am using HepG2 cells as the model system

31 December 2016 9,575 1 View

Can someone suggest E2-inducible gene in HepG2 cells as an internal control?

I am using HepG2 cells as the cell model. I am giving treatment of estradiol. But i want to be sure that my estradiol drug is dissolved correctly. so anyone can suggest me a known E2 inducible...

31 December 2016 2,288 0 View

How to decide time points of RNA extraction in microarray experiment?

Hello Experts, How do we decide time points of RNA extraction after giving drug to the cell lines for checking the gene expression profile in a microarray?

31 December 2015 6,113 6 View

Can I use the half life of the drug as the time point?

I don't know the time since all individual genes will be expressed at different time points. Can I use half life of the drug as the time point?

31 December 2015 6,190 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View