Please suggest me an inducer of PPAR alpha protein expression?

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Can you please suggest a simple and easy protocol for Medicarpin estimation?

Medicarpin estimation requires a long protocol and HPLC. Is there any other way Medicarpin can be estimated? Your help will mean a lot. Thank you.

06 September 2022 589 0 View

Do antibiotic resistance gene primer is same for all bacteria?

I need a suggestion on different antibiotic resistance gene. Is it same for all bacteria?

16 May 2021 7,888 3 View

How to find which group has higher significant difference than the other group?

I have a dataset that has two groups: mean_1 and mean_2. I want to find statistical significant difference between mean_1 and mean_2. The groups are paired but data is not normally distributed so...

17 February 2021 8,697 12 View

Has anyone here used NEXRAD Data to access historic hourly precipitation data?

I've trying to access hourly precipitation data from NCEI's Radar Archive however I've not been able to configure past the downloads. Is there any code available to decode the data?

15 February 2021 6,861 1 View

How do we calculate 95% confidence intervals for RMSE?

I have two datasets (measured and modelled). I want to calculate 95% confidence intervals for RMSE. Cany anyone please help me with this? Thank you in advance

04 February 2021 6,094 6 View

how to analyse the impact of independent variable on multiple dependent variables?

I have a dataset which three variables: date, mean1 and mean2. date - datetimens64 (YYYY-mm-dd format) mean1 - float type mean2 - float type The data is not normally distributed. I want to find...

19 January 2021 8,391 5 View

Which is the best machine learning regression model to analyse small data sets?

I'm trying to predict mercury concentration in soil with a data set of 67 observations with 28 predictor variables. Some of the predictor variables are correlated.

05 July 2020 7,455 5 View

Is 10 years of data enough to study trend of stream discharge and precipitation?

I'm studying to study hydrological regime of a watershed for a statistics project and I'm wondering if running a Mann-Kendall test would be useful for the analysis of these data set.

15 April 2020 9,547 35 View

Why iron sulphide sediments are frequently formed during winter and role of microorganism in formation of iron sulphide mineral?

I read in a book Environmental Chemistry that" in anoxic region of water bodies , some bacteria use sulfate ion as an electron receptor where as other bacteria reduce iron(III) to iron (II). These...

22 October 2019 9,029 3 View

SiRNA mediated knockdown is observed at RNA level but not at the protein level. What might be the reason?

I am currently doing siRNA experiments and getting 80% knockdown at RNA level, however no effect is seen at the protein level even at higher concentrations tested. What more should i try?

04 August 2019 9,686 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View