Gluthation Transferase (GST) and Glutathione Redu?

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Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

21 May 2024 9,951 2 View

Need a research topic related to GST?

I need a research topic related to recent trends in GST

16 April 2024 1,805 0 View

How to expression and purify full length TRAF2 protein from bacteria?

Hi, I am for the first time struggling with TRAF2 full length protein purification from BL21 or Gold but I fail to purify it although I tried GST, MBP and His tag it the N- terminal. Do any...

07 April 2024 1,090 0 View

How to calculate (tot) glutathione peroxidase activity (GPx)?

Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...

03 April 2024 9,344 0 View

Should I use the supernatant or lysate part of the cell?

When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...

24 March 2024 5,247 2 View

Multiple primer sequences in sequencing result after PCR?

I was doing site-directed mutagenesis using PCR. And I have ordered forward and reverse primers that are reverse complements of each other (both primers are around 45bp, 7 aa before and after my...

19 March 2024 5,082 0 View

What is the appropriate conversion factor to convert the units of measurement for antioxidants from Micromol/L to international Unit?

A For dear researchers, we have a group of variables that were obtained in different units, which are the enzyme superoxide dismutase , Glutathione and Malondialdyhyde in micromol/liter and...

18 March 2024 5,293 3 View

Why Marketing and ad industry is loosing employees in India ?

Considerable points can be AI, Funding winter, Demonetisation, and GST. But we are open to other considerable reasons.

04 February 2024 959 1 View

Why my GST fusion protein is eluted out in wash buffer and flow theoigh compared to elution buffer?

Hello everyone, This is my first question and very curious to learn why my GST fused protein shows band in the cell Lysate flow through, lysis buffer, wash buffer, but absolutely no bands on the...

25 January 2024 7,470 3 View

Does purified GST recombinant protein have function by added in cell culture?

Hi, everyone. I resently made the expression and purification of a GST recombinant protein. I am wondering whether this protein can have its function by added in the 293T or HeLa cell...

25 December 2023 10,012 2 View

Juan Manuel Gallardo

May be this insert help you,

https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

Mohd Akram Ansari

Article Over-expression of chickpea glutaredoxin (CaGrx) provides to...

Kindly go through the above-mentioned research article for detailed GST and GR enzymatic assays.