Hi everyone, I'm pretty new in lab, and I have a question I did transduction in a neuronal cell line with PINK1 WT and mutation L347P, but we didn't titration viruses because that moment, we didn't have the materials for titration, only we use the same volume of viruses (6uL) for both groups, (Now I know that titration is necessary to know if we put the same amount of viruses)

We did a Western blot, and the expression protein was higher in WT regarding the Control, and lower in L347P mutation than control. We measured other proteins like GAPDH (housekeeping), LAMP1, and Calnexin, and there aren't any differences between any group

after that, we made an RT PCR, and I realized that the differences between mRNA in mutation are almost double than WT, so... I don't know If I can use that data because the amount of mRNA levels are very different, on the other hand, I think that if there aren't differences in the expression of other proteins belonging to other organelles, maybe there aren't other alteration in cells

I'm searching bibliography about lentiviral transduction and expression mRNA levels but I can't find any information regarding my question, I appreciate your help