How to fabricate a thin layer of PEI film without defect?

I want to cast a thin layer of Polyetherimide/DMAc dope solution with thickness of about 50 micron on a glass to fabricate an ultrafiltration membrane via phase inversion method. Can any one give...

14 August 2020 247 3 View

What is the best approach to screen co-expression of a list of genes in diffrent bacterial strain?

Hi, I am wondering what is the best experiment (cheap and fast) to screen co-expression of a list of genes in different bacteria strains? Lets say, we have around 100 bacterial strains and for...

10 August 2020 7,583 3 View

How can we reduce PEI porous membrane shrinkage?

We are producing PEI membrane as support for gas separation membranes via phase inversion method by casting solution of PEI/NMP and water as coagulant. We cast on polyester support but membrane...

14 June 2020 2,324 1 View

Why COMSOL's particle tracing module is not accurate and reliable for inertial focusing?

Dear researchers, I am working on developing a reliable and accurate COMSOL model to investigate the inertial focusing of particles inside micro-channels. Although I add and define the lift...

09 May 2020 797 2 View

What can be joint projects between chemistry and mechanical engineering?

how can I find a common related project between chemistry (polymers or biomolecules) and mechanical engineering( for example fluid mechanics )???

15 April 2020 2,388 1 View

Best novel method to optimize 3D geometry and its fluid?

Do you have any idea what is the best method to optimize 3D geometry and its fluid without linking the code with fluent (or any flow solver) ? In order to find the best 3d geometry and decrease...

10 April 2020 9,321 1 View

How can I predict the road lanes if I have the system that detects the lanes?

I have used the code in this repository linked below and implemented a lane segmentation system: https://github.com/MaybeShewill-CV/lanenet-lane-detection Then I found this demo which is Tesla...

17 March 2020 2,924 3 View

I am working on a hand gesture recognition project. I am looking for a good dataset. Does anybody know where to find?

Hi researchers all over the world. I am working on a simple detector which can annotate the hand gestures and also is able to recognize the differences between each gesture using the CNN...

16 March 2020 9,055 3 View

How can I find a dataset for lane PREDICTION?

I want to work on a Lane Prediction model. I already worked with dataset that has segmented lanes like Culane, but I am looking for a dataset which has predicted lanes. for example this is a demo...

27 February 2020 8,633 8 View

How to Detect autophagy using Acridin orange?

I have a problem interpreting the results of acridine orange by flow cytometry. Do we see red fluorescent in cells with increased autophagy and green fluorescent in suppressed ones? And if so,...

20 January 2020 8,245 1 View

Where can I obtain Streptomyces lividans strains for enzyme production?

I am having trouble figuring out where to obtain Streptomyces lividans strains that are commonly used for enzyme production. Specifically I am searching for the following strains: S. lividans...

25 May 2020 6,205 3 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

05 February 2020 1,232 3 View

I need to extract secondary metabolites from Actinomycetes, can anyone help me ?

Hi, i'm workint now on Actinobacteria (Streptomyces) cultured on ISP2, and i need to extract it's metabolites to reveal its insecticidal effects, can anyone guide me which protocolor or procedure...

01 February 2020 8,450 3 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

01 February 2020 8,399 3 View

Could anyone help me to identify which is mouse crypt of this picture below?

11 December 2019 6,115 3 View

Which one is better citrate buffer or Na-phosphate buffer?

I want to work with Streptomyces sp as whole organism and its crude LPMO (lytic polysaccharide Monooxygenase) for sugarcane bagasse pretreatment for bioethanol. Which one is better to use and...

09 October 2019 6,174 3 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

08 September 2019 7,719 2 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

17 July 2019 5,854 2 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

06 July 2019 5,350 2 View