I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
May be this insert help you,
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf
Good day!
Using a plate reader or a UV/Vis spectrophotometer, the conjugate formed by the GST-mediated reaction of CDNB with glutathione is measured. Additional information, the normal ranges for plasma ALT and AST were 0–40 U/L and 0–37 U/L, respectively, for GST. Using a quantitative enzyme immunoassay, the level of alpha-glutathione S-transferase (-GST) in plasma was determined. Set the spectrophotometer to 340 nm to prevent a negative value. On a kinetic program, read after a lag of one minute every 30 seconds for five minutes.
The attached link below might help you perform the proper/accurate procedure for measuring a GST activity.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf
Hope this helps. Good luck!
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