Hi
I am using c1740 - c1q - and testing it in WB - I use anti c1q-c.
Human complement C1q has a molecular mass of 460 kDa. C1q is composed of 18 polypeptide chains, 6 each of 3 different polypeptide chains (A, B, C).
The A, B, and C chains have the following individual molecular mass values, following analysis by reducing SDS-PAGE: A-chain: 31 kDa , B-chain: 29.7 kDa C-chain: 26.1 kDa.
the size I got in WB is 25 ( C-chain)