it wil be helpful to get ctab extraction buffer components needclike how much pvp .
Hello Sreshtha Das
In general, EDTA, CTAB Buffer, Sodium acetate buffers and Tris HCl are all autoclavable but please ensure you do not have any other heat labile components added to them before autoclaving. Incase of any requirement of heat labile components to be added please ensure you follow filter sterilization to remove any contamination.
Neeraj Kalyan Gandholi, could you please specific heat heat-labile components you are mentioning, specifically in the plant tissue culture or bacterial, or yeast culture.
Correct me if I am wrong, most salts/buffer can be autoclaved right?
Sreshtha Das some of the heat labile components could be enzymes RNase A, Proteinase K, Phenol (for phenol-chloroform extraction).
Answer is yes to your second question. Most buffers can be autoclaved as they are prepared by dissolving salts in water.
Hope this answers your questions
Neeraj Kalyan Gandholi i am on the right track because enzymes are proteins and that enzymes get denatured at high temperature , however the temperature differs
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