Extraction of alkaloids, tannins, and steroids from plants typically involves solvent extraction using acidified or organic solvents followed by concentration and purification. Quantification is commonly done via spectrophotometric assays and chromatographic techniques, with method specifics optimized based on the compound's polarity and plant matrix.

Extract the plant with hot or cold ethanol will extract most of the compounds because ethanol is a general solvent. Second step is partitioning after concentration of the ethanolic extract and add water yo make the organic solvent immisible with the total extract. Hexane will take the non polar compound then ethyl acetate will take the intermediate polar compound. The remainder is the polar compound