In our current project, we're working with the Rapid SARS-CoV-2 Antigen Test Card and have incorporated polyethylene glycol (PEG-3350) at a concentration of 68 mg/mL into the running buffer. Unexpectedly, we've observed a significantly brighter test line even when no antigen is present in the sample—only the processing solution and PEG-3350 are included.
We suspect this phenomenon may be due to non-specific binding or interactions between PEG-3350 and components of the assay.
Has anyone encountered similar effects when using PEG-3350 in lateral flow assays? What strategies or modifications have you found effective in mitigating such non-specific signals?
Any insights or suggestions would be greatly appreciated.
Hello, Amit Kumar Acharzo.
Q: 68mg/mL of PEG-3350 induce a test line signal in a lateral flow assay without antigen?
Nonspecific binding is likely to occur.
It is recommended to add blocking agents such as bovine serum albumin (BSA) and casein to the buffer, which can occupy nonspecific binding sites on the test card.
If you have any other questions, please contact me.
Lucian Miles I tried only 1% BSA, but it didn't work. Could you please suggest to me, do I need to try both BSA and casein? If I do, what percentage should I use? I am grateful for your assistance and encouragement.
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