What should be boundary condition on wave function in a continuous potential?

If the potential is a continuous function of x ,say V,(x)= - A ( exp(2|x|/a) -1) ,what should be boundary condition on wave function ? Further ,will it be correct to argue ,wave function and...

11 December 2020 6,617 4 View

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA?

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA? The OD values obtained at different dilution how to determine the cut off OD against which the antibody titre...

31 January 2020 909 4 View

DNA precipitation without ethanol?

Is there a way of precipitating DNA from water without using ethanol? for example, mixing DNA with glycogen and ammonium acetate and then spinning it long enough so the bound glycogen+dna would...

22 August 2019 5,501 6 View

What is the meaning of writting a comment on a published paper ?

When one writes a comment on a published paper , what is actually the intention of the commenting author ? To point out the mistakes or present complete solution . It is seen that very often...

25 February 2019 6,029 4 View

Multiple peaks of the same compound in LC-MS Analysis?

Dear All, I was analysing some MAAs (mycosporine amino acid like substance) samples. HPLC shows a single peak with RT 4 wavelength 333nm. so I thought it is a pure compounds. To know the mass of...

21 August 2018 9,698 3 View

Silicon atom types in Charmm36 ff?

Hi, I want to simulate a ligand containing Silicon atoms ( present in the aromatic form as well). I wanted to ask if anybody could let me know the presence of silicon atom types in Charmm36...

14 June 2018 4,648 3 View

Can I use PVP instead of Sodium acrylate for the synthesis of graphene oxide magnetite nanocomposites?

It says that sodium acrylate is used as a stabilizer and to improve solubility of the precursors. And without its presence the particles aggregate. Can I use PVP instead??

12 June 2017 7,979 3 View

Can you help in MATLAB for H=p^2 - x^4 +2ix on eigenvalues ?

Can you help me in eigenvalues calculation using MATLAB for H=p^2 - x^4+2ix ?

29 June 2016 5,501 2 View

What is an index value in a gray scale image which is not in an rgb image?

When i convert an rgb image to a gray scale image in MATLAB, there is an index value in the gray scale image but for the rgb image.

03 April 2014 1,831 1 View

Why we are reluctant to admit own mistakes ?

It is seen that some people are reluctant to admit their own mistakes . Do they feel their social dignity/ prestige are in danger ? On the other hand they should know that one can make fool to...

01 January 1970 8,902 63 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View