I am looking for a vacuum pump to infiltrate solutions into plant tissues in order to fixate them (for molecular biology experiments like ChIP, in situs, etc…).I need something powerful, because it is not just for Arabidipopsis seedlings, but not too strong and there are plenty of options in the market ¿How much vacuum power do I need?
Hi, Pedro! I don't know what type of tissue and species you're working on, but I'm currently working on sunflower seedlings for some chromatin related experiments for another group, and it worked quite nicely using a bomb of 7 torr (9mbarr)...
I hope it helps =)
Hi, this is the one I use:
http://www.ebay.com/itm/Edwards-E2M8-8-Rotary-Vane-Two-Stage-Vacuum-Pump-Works-/181245934118
Yes, usually it is used a rotary vane pumps. But I learn that maybe a stroger vacuum is better (about 2 x 10-3 mbar / 1.5 x 10-3 Torr)
Thanks.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology