You could use --Herbert et al,1971--METHODS IN MICROBILOGY,CHAPTER3,ACADEMIC PRESS .this method is used routinely by my students for the determination of soluble proteins.
Hi Sharma,
it's may depends on your method of extraction but in general Bradford assay is a good protocol to quantify proteins in plants (independent of your extraction).
I frequently use Bradford assay with a series of concentration of BSA as standard curve to measure protein levels on leaves.
I think this protocol that I linked is a good one.
Best regards,
Vitor
http://cst.ur.ac.rw/library/Food%20Science%20books/batch1/Food%20Science%20and%20Technology%20Published%20articles/Analysis%20Protocols/The%20bradford%20method%20for%20protei%20quantitation.pdf
Dear researchers,
Thanks much for your valuable suggestions.
Best regards,
Anket Sharma
Dear
It is not my field but I think this article will be useful for you
http://rd.springer.com/article/10.1007/BF01015193
regards
Hi,
Depending on your extraction process, the GE Healthcare 2D Quant Kit may be useful - it is expensive, but it is not affected by the chemical composition of your buffer (many common buffer components, especially detergents and salts for extracting difficult proteins , impede the colour of the Bradford and BSA assays).
You can find the GE Helathcare kit here: http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences-ca/80648356
Hello Anket
It depends on the method which is followed for extraction, quantity of sample available with you and more importantly objective of protein estimation. All commonly used protein estimation methods have merits and demerits. For Biochemical estimation of enzymatic assay you can go for BCA and Bradfford methods.
you may find this web link useful
http://bitesizebio.com/23824/top-5-protein-quantification-assays/
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