During plant tissue culture researcher using citric acid and ascorbic acid to minimise the browning problem of explant. Under which mechanism these two compounds prevents tissue from browning .Please if references provided will be appreciated
Both substances, citric and ascorbic acids, are powerful reducing agents. In this way they compit for the oxiation of phenolics. From a practical point of view, I had never succeed with the reduction of phenolic oxidation in tissue culture by mean of the use of citric and ascorbic acids, but this's an opinion because some other factors should be considered: the plant specie, the tissue and organ cultured, their phonologic age, the culture conditions.....
If you want to know the details of mechanism of controlling of browning in tissue culture , you can read the Book , Plant Tissue Culture by S.S. Bhojwani M.K. Razdan.
The effects of ascorbic acid on lethal browning and callus formation have been well studied. Here are the references:
Article Control of lethal browning of tissue culture plantlets of Ca...
Article Control of lethal browning by using ascorbic acid on shoot t...
Article Effect of ascorbic acid concentration on structural characte...
Article Effects of Ascorbic Acid in Controlling Lethal Browning in i...
Please read the attached paper to get more information on tissue browning.
Article Inhibition of phenylpropanoid biosynthesis increases cell wa...
acorbic acid does not compete for the oxidation of phenolic compounds. it is not a competitive inhibitor. instead, Ascorbic acid acts as powerful scavenger that inhibits intermediary products of the phenolic oxidation; in addition ascorbic acid inhibit polyphenol oxidase activity, the enzyme which catalyses the oxidation of phenolic compounds preventing therefore tissue browning
Dear Muhammad
Ascorbic acid is a good antioxidant and the most antibrowning agent widely used today. Some reports attributed its action to the reduction of the formed quinones to the original colorless diphenols; others considered ascorbic acid as a PPO inhibitor; however, the condition required to possess each mechanism is not defined. Polycaboxylic acids e.g. citric acid act as PPO activity either by lowering the pH or chelating the copper at the enzyme active site.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology