Has anyone got an idea about separation of inner and outer chloroplast envelope membranes from Nicotiana benthamiana?

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Vandana Tomar

Thank you sir..we have been trying the same protocol for a while, but unfortunately couldnot fractionate the envelopes. We could isolate the intact chloroplasts but the envelope membrane fractions are not visible in the sucrose gradient. We donot use a gradient maker for generating the gradient. Is the gradient a reason ?