Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

What a 50% positive predictive value mean?

I want to explain a threshold of 50% PPV to describe how some assays perform when the prevalence of disease is high or low

07 October 2020 8,657 6 View

Author who is not actually an author of some of my publications?

I came out to realize that a person who is enlisted as an author of some publications that I am the REAL AUTHOR, is marked instead of me. How do I change that? Example: Standardized sampling...

21 September 2020 378 7 View

How to increase the short circuit current density in a perovskite solar cell?

I'm optimizing the procedure for making inverted PSCs: ITO / PTAA(8nm) / CsMAFA(320nm) / PCBM(60nm) / BCP(9nm) / Ag(100nm). The cells look very good, I have a very good FF (0.70) and Voc (1.0V),...

16 September 2020 6,286 8 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

Sensors and Systems for IIoT?

13 March 2020 7,295 3 View

Does someone know the cause of stains appearing on n-i-p Perovskite Solar Sells?

I did the procedure described by saliba ( How to Make over 20% Efficient Perovskite Solar Cells in Reg... ) to make n-i-p PSCs. However, after perovskite deposition stains appeared in the cell...

29 October 2019 8,012 3 View

How to process small samples for histology?

Hi, I have very small samples of about 1x2mm (dental pulp tissue) and I need to process them for histology (dehydration, parafine inclusion, stainning with HE, etc). The problem is that the...

18 September 2019 1,180 4 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View