What is actually happen when I got band detected on gel electrophoresis at 10,000 bp? - FAQS.TIPS

Syafiqah Mhd Jamal @Syafiqah_Jamal

12 December 2018 1 6K Report

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations with the volume of primers that I used?

I did 15ul total for each samples, and I used 5ul of DNA template for each samples. Any suggestions on how to troubleshoot this problem?

Thank you all.

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Science method

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