Polymerase Chain Reaction

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in Polymerase Chain Reaction to get answers

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Questions related to Polymerase Chain Reaction

What is the reason for fertilizer use efficiency increased with decreased fertilizer levels?

Suppose their are different fertigation levels 50% RDF, 75% RDF, 100% RDF and 125% RDF Fertilizer use efficiency for 50% was 115 kg/kg NPK, for 75% 102 kg/kg NPK, for 100% 95 kg/ kg NPK and for...

28 May 2024 6,242 4 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

22 May 2024 4,912 4 View

Why in myloid leukemia the wbcs increase in number while Rbcs and Platelets decrease in number ?

What is the cause of increasing only WBCs while RBCs decrease although it is the same line in erythropoiesis

02 May 2024 7,965 3 View

Are my sequences too long of PopART?

I am trying to make a haplotype network with popART, for the haplotypes instead of choosing one gene, I am adding together to alignements of related genes. This gives me an alignement of 5.7Kbp....

30 April 2024 2,735 0 View

How can I get involved in writing a chapter of scientific book in my research area?

Any lead will be helpful

25 April 2024 5,878 3 View

Can someone please explain how the Public Health/ Community Medicine works in United Arab Emirates (UAE)? What'r diverging/converging specialities?

Is Public Health/ Community Medicine similar in the UAE? What are the domains of Public Health / Community Medicine? What are the different diverging branches converging in this mainstream? What...

24 April 2024 9,767 1 View

Does anyone have experience of time-lapse microphotography of dividing bacteria?

I am interested in producing videos of growing and dividing bacterial cells. The problem, as I see it, it to keep the cells still and well-oxygenated without them drying out. I have tried using a...

22 April 2024 7,110 2 View

On , My09/11 and GP5+/6+HPV L1 region sequencing for genotyping primers for Nested PCR used but why I couldn't find sequences other than 16?

For my thesis on the same HPV L1 region sequencing for detecting genotypes , I have used My09/11 & GP5+/6+ primers for amplification on Nested PCR, but I couldn't find sequences other than 16,...

20 April 2024 1,686 1 View

What is the physical reason that hydroponic plants can be planted closer together then soil-based plants?

In other words, is it simply the nutrient supply that makes soil plants much less effective when they are closer together or does it have to do with the roots interfering with each other. If it's...

17 April 2024 6,652 4 View

How can intramuscular preadipocyte be isolated from rabbits?

Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit

17 April 2024 9,346 0 View

What is the best way to concentrate the protein and remove the imidazole post Ni-NTA purification?

I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...

17 April 2024 5,664 0 View

Calculation of insecticide concentration on WHO impregnated paper?

Need to calculate the concentration of insecticide on impregnated WHO paper in microgram/ml.

03 April 2024 5,427 0 View

Why my PCR product is too short?

Hi everyone, I need your help with optimazing my PCR reaction. My PCR product should be 850bp. Starters Tm is 65, they do not form dimers or secondary structures and they’re specific (Blast...

16 March 2024 2,592 2 View

DdPCR 3' non extendible probe query?

In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...

15 March 2024 6,947 1 View

Why can't the fructose monomer in sucrose be α-fructose?

In all academic sources, sucrose is identified as α−glucose (1-->2) β−fructose. However, I cannot find any explanation anywhere as to why the fructose monomer has to be in the β configuration....

13 March 2024 9,156 4 View

Can anyone suggest me how to ensure proper revival for the strain B. braunii UTEX 572?

I have bought strain B. braunii UTEX572 for my work, however, I do not see any positive growth after reviving it in both Bold 3N liquid and agar media supplemented with 10g/L glucose. Is there any...

11 March 2024 4,128 0 View

What is the role of artificial intelligence in advancement of agriculture and environmental significance of organic carbon?

06 March 2024 6,074 2 View

Can you get same values of markers of T cell activation for same samples by using BD FACS Canto II and Cytoflex by Beckman Coulter?

Two different cytometers will give same or nearly similar results for activation markers on lymphocytes?

05 March 2024 998 1 View

Are native precast gradient gels good for EMSAs?

I am wanting to switch from self-cast to pre-cast gels for my EMSAs. I am currently trying to decide whether to buy a constant percentage gel (eg. 6%) native gel or whether to try a 4-16% gel for...

04 March 2024 7,913 0 View

How purification exosome from primary human NK cells and CD4+ T cell ?

Greetings I possess a keen interest in exploring the potential of primary human CD4+ T cell and NK cells-derived extracellular vesicles. Currently, I am in the process of formulating a research...

26 February 2024 3,373 0 View

Western blot transfer time?

Hi, I'm an undergraduate student working on western blot. I have understood most of the principles of western blot but still have some questions about it. 1) I wonder why the transfer time is...

23 February 2024 4,909 0 View

What could be the reason for no protein expression in a tet-on doxycycline inducible system in mice?

I am treating tet on mice with doxycline (76ppm) for 2 weeks and i dont get the overexpression according to Western Blot. In rtPCR i see differences, but in protein level these differences are...

21 February 2024 8,160 1 View

How did the Sysmex DI-60 digital morphology analyzer contribute in the field of Medical Laboratory Science? What are its benefits and disadvantages?

This question is based on the article, "Red and White Blood Cell Morphology Characterization and Hands-on Time Analysis by the Digital Cell Imaging Analyzer DI-60".

21 February 2024 7,893 3 View

Quais as implicações do uso da metodologia de histórias de vida para pesquisas sobre uso de substâncias psicoativas ?

A metodologia de histórias de vida conseguem abarcar nas falas dos interlocutores elementos para compreensão do uso de substâncias psicoativas, traduzindo a subjetividade de como pensam, sente e...

15 January 2024 4,529 0 View

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