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Questions related to PCR Analysis
I extracted human embryoid body RNA for PCR analysis using Trizol reagent. RNA conc. is between 50-200 ng/ul, and 260/280 ratio is about 1.7-2.1,so these are really good, but 260/230 ratio is...
09 September 2014 4,368 6 View
I got 260/280 ratio between 1.8-1.99, Also I got 260/230 ratio around 0.6-0.95 and concentration is around 390-400 ng/ul (RNA dissolved in DNAase/RNAase free water). I want to run rtPCR and...
09 September 2014 1,691 4 View
We are looking for an automated electrophoresis system similar to Agilent 2100 Bioanalyzer for determining the size of fragments amplified by PCR. We would like the system to work with 96 well...
09 September 2014 5,333 3 View
A few months ago I got my PCR product, but now I cant repeat it with the same DNA and the same PCR instruction. There are a lot of non specific bands in my pcr product and i cant remove them with...
08 August 2014 5,574 13 View
I am not getting amplification of my gene. Its gc content is 53 % do I need to use supplementary substances like DMSO etc, its Tm is ~62 degree. the primers have been designed with restriction...
08 August 2014 5,415 13 View
This question is probably mostly for statisticians, but here we go. It is easy enough to serially dilute DNA (or cDNA, RNA...) to a theoretical concentration of 1 copy per uL of water. My question...
08 August 2014 7,605 3 View
I had added MgCl2 buffer twice in PCR master mixture & unable to find any PCR Product.
08 August 2014 2,097 4 View
Likely I need to get bands at
08 August 2014 5,445 7 View
I had done PCR in two batches. I had casted a agarose gel using two combs. I had loaded few samples from the first batch (most of them had been already visualized and had given good amplification)...
08 August 2014 1,982 8 View
After using the qPCR days before for a multiplex reaction, I created another run template for a singleplex reaction and set the thermal profile. As I started the reaction, an error message from...
08 August 2014 3,201 3 View
I am going to measure the expression of some genes in the spleen of asthmatic mice. Given the various reference genes that have been indicated in papers, which housekeeping gens do you think best...
08 August 2014 808 4 View
I've been using 16S universal primers to amplify bacteria DNA (515F and 806R), but I found faint band in the negative control. I did different combination of reagents, including changing water,...
07 July 2014 5,637 7 View
I have sterilized the working lab, autoclaved the plastic wares and have also changed the reagents like buffer, MgCl2, DNTP, Taq, water etc. I am generally working in Laminar flow hood.
07 July 2014 1,777 8 View
I would like to detect the presence of hookworms species in the feces samples by PCR. However, most of current protocols use the commercial kits. My laboratory can not afford to buy KIT. So, does...
07 July 2014 8,304 3 View
Here, I did long-range PCR for ranges between 8-24Kb. I used 0.4% agarose concentration but the PCR stuck to the wells and some of the parts migrated. I am not sure if this is the same products...
03 March 2014 4,335 10 View
I have PCR products that I had to dilute to define the DGGE bands, but how can I compare these lanes? With the next statistical analysis?
02 February 2014 6,541 2 View
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serum samples
01 January 1970 7,584 0 View
Hi dear Dose someone has using multiplex PCR analysis for DENGUE and CHIKUNGUNYA or both simultaneously? Please give me information about the reagen or protocol for co-analysis of them
01 January 1970 6,556 3 View
can I amplify FOXO1 and RUNX2 with proof reading polymerase such as high-quality Taq polymerase. Then, incubate the PCR product with ordinary Taq polymerase to generate A overhang Also, I would...
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