How to increase the intensity of bands in sqRT Pcr analysis for gene expression?

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How to improve RNA extraction using Trizol?

I have been extracting RNA using trizol method from plant tissues but sometimes during the extraction procedure there is low pellet formation by which the quality and quantity of RNA gets...

09 October 2018 9,101 0 View

What might be the reason that during quality using 0.8% gel electrophoresis i got a single thick band but not two separate bands as its seen normaly?

I extracted RNA from the fresh tissues using RNA kit form GCC Biotech, normally during the Quality check using electrophoresis it showed Double band but today in my case i could get only one...

08 September 2018 3,595 4 View

Can someone suggest me what might be the possible reasons for the appearance of smear, faint and no bands in semiquantitative RT-PCR gene expression?

I use the primers synthesized by molbiogen and all the pcr components purchased freshly and cDNA synthesized shows expression in housekeeping gene. I tried in normal DNA i got faint bands for the...

07 August 2018 7,707 2 View

Does housekeeeping gene show two different band size when the cDNA is prepared using reverse primer instead of Random Hexamerase?

I extracted the RNA and prepared cDNA using the reverse primer of OsActin instead of Random hexamerase and did a PCR for the same, but to my surprise i found two bands of different size of two as...

07 August 2018 462 5 View

Is it possible to do acid digestion of plant material in big borosil testube using water bath optimizable to 100 degree celsius?

Acid digestion of rice roots in 70% conc. HNO3.

05 June 2018 757 2 View

Please can anyone suggest is it safe to keep RNA in -20 degree celsius for some days instead of -80?

i wish to store RNA after isolation temporarily in -20 degree celsius is that possible and how safe it is?

04 May 2018 4,773 3 View

Please Can anyone suggest me a method of powder sample preparation from rice root tissue for XRD analysis of presence of elements?

I wish to study the presence of elements in rice roots under heavymetal toxicity.

03 April 2018 1,673 2 View

Reasons for the use of CaCl2 solution in preparing Aluminium stress solution after growing in nutrient solution. why do we use CaCl2 solution?

01 February 2018 3,764 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View