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Questions related to PCR Analysis
Here I try to make out Panther analysis for highly expressed genes detected in Realtime PCR analysis? will it be possible? or any method can be suggested to make gene clustering? Thanks in advance.
11 January 2017 5,090 1 View
I'm using white adipose tissue and brown adipose tissue for real time PCR analysis and future analysis of protein expression of PGC1a and UCP1 (using Western blot analysis). I've only had...
01 January 2017 1,733 1 View
01 January 2017 5,931 1 View
Hi, I will work for quantfication of miRNA in plasma samples with real time TaqMan microRNA analysis. First of all, for extraction of miRNA, i choose (miRNeasy Serum/Plasma Kit For purification...
30 December 2016 2,596 7 View
I have isolated R. solani from the soil. I have studied morphological studies of R. solani. Now I want to conform its strain further by PCR. Suggestions are needed.
12 December 2016 9,999 8 View
We need to get the bacteria from the blood of patients with sepsis for diagnosis, is there any commercial device or prototype capable of efficiently purifying bacteria from blood sample (5-7ml)?
12 December 2016 8,824 4 View
To increase the sensitivity for PCR methods of detection of microbial foodborne pathogens, the bacterial numbers are normally enriched from 5 hours to overnight. Is it possible to reduce this...
12 December 2016 4,878 3 View
DNA is methylated, but when it is amplified why does it lose its methylation? I'm asking this in connection with the use of DPN1 enzyme which is used to distinguish between template DNA and...
02 November 2016 5,335 7 View
Can anyone suggest a citation to support/refute the the relationship between DNA concentrations and erroneous PCR product? I am struggling to find peer-reviewed resources that speak to this...
26 October 2016 5,248 3 View
Hello everyone I would like to get suggestion from you people regarding the reaction volume of PCR for my q PCR analysis. I have already cheked my cDNA by actin/18s primer in 10 microlitre...
09 September 2016 10,087 3 View
Recently I saw that the Sol Genomics webpage has taken down the option of doing In silico PCR for Arabidopsis. While I have found many programs that allow you to utilize an input sequence, these...
08 August 2016 9,558 4 View
I am assessing the effect of Thermotherapy on Citrus Huanglongbing pathogen in root zone. PCR test result is still positive but I am not sure if there are any approach to identify if the pathogen...
06 June 2016 4,441 11 View
I am doing RT-PCR and i need to detect RNA as low as 10 to the 1 copy number. These are my positive controls as my final detection level will be this low in my serum samples.
06 June 2016 3,107 4 View
01 June 2016 6,183 11 View
Gene A control null ct is 24.76 and my kd down gene ct is 24.2 GAPDH control 20.7936306 gene 19.23660088. I am calculating 50% knockdown. but other s considering there is no knockdown based on...
25 May 2016 8,575 4 View
I am currently trying to analyze my RT-qPCR data and I am hearing differing methods on how to properly perform the analysis. I have 3 technical replicates for each of my biological samples and I...
10 May 2016 5,223 3 View
I eluted my amplicon from gel and checked its concentration after elution (column based kit) on 0.8% agarose gel. In the eluted product I found another band above my amplicon, which is very...
27 April 2016 6,015 13 View
I am unable to understand the representation of the data. What does 10,0 and 0,1 or 1,0 represent? Description on the paper says that Akt levels remained almost unchanged in Hek 293 cells ? How...
03 March 2016 3,826 3 View
How can i determine antimicrobial resistance genes for pcr analyses? Are there many of antimicrobial resistance primers for one genes (ex. floR)?
11 February 2016 8,212 2 View
I am doing PCR with GJB2 primer regarding hearing loss. When i dilute the primer for the first time from the stock, it works only for 2 days. After it it does not work even i dilute it again from...
08 February 2016 5,832 3 View
I am trying to amplify a vector by pcr. I linearised the vector with EcoRI and BamHI to ensure no re-ligation. I then purified the linearised vector and used that in my pcr reaction. I am...
04 February 2016 9,647 3 View
02 February 2016 8,015 2 View
I am runing two steps RT-PCR and the target size is 2.2kb. However, my products cannot migrate when I am runing the gel. I measured the concertration of my cDNA using nanodrop and it is around...
02 February 2016 1,937 5 View
01 February 2016 5,475 4 View