What are the best housekeeping genes for qPCR analysis of Arabidopsis root under nutrient stress and brassinosteroid hormone treatments?
A.o.A.
For Arabidopsis roots, i used "Actin" as housekeeping gene and its very good. I didn't performed the other treatment. But i think you can also use the same (actin) for the other purpose. There many other housekeeping genes like GAPDH, tubulin, cyclophilin A, ribosome prots, etc. ). You can go with more than one too.
As mentioned before, actin and tubulin are generally stable housekeeping genes. Additionally, in our lab we often use APT1, which seems to be extremely stable in any tissue/treatment/stress we study. Whatever you choose, make sure it is not affected by any treatment/tissue type.
Always good to go with more than 1, or even use the means of more than 1 to calculate your dCT.
Hope it works out.
We used UBQ10, 18S rRNA and Actin for many experiments including BR and nutrient stress in Arabidopsis. We found a very stable expression of all these three genes across treatments and tissue types. As Sjon Hartman mentioned, it is always good to use more than one housekeeping gene for qPCR experiments.
Good luck with your experiments!!
Beautiful question and great answers
I wish you success in the service of humanity and science
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