I know that DNA adsorption to plastic container surfaces (like polypropylene) can result in loss of sample, but I wanted to know specifically how much. I know this depends on several factors, like time, temperature, formulation, etc.. I was hoping I could get some ballpark numbers.
Can anyone point me to a paper or in the direction of where to look up the actual quantity of nucleic acid (ssDNA and dsDNA in particular) adsorption to different plastic polymers and/or silica types?
The best I found was this reference: Gaillard, Claire, and François Strauss. "Avoiding adsorption of DNA to polypropylene tubes and denaturation of short DNA fragments." Technical Tips Online 3.1 (1998): 63-65.
I agree that "Avoiding adsorption of DNA to polypropylene tubes and denaturation of short DNA fragments." Technical Tips Online 3.1 (1998): 63-65 is the best paper I also know.
There is described ~25% reduction of DNA concentration using polypropylene plates.
Thanks for the reply! But that 25% reduction was only with really low concentrations, right? I'm assuming the limiting factor would be surface area of the plate?
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