Dear all,

I would like to know if any of you have experience in dephosphorylation of proteins using Shrimp Alkaline Phosphatase. I am currently buying lambda phosphatase, but since I had SAP in the lab I tried it and had nice results. But... What does these results actually mean?? That's the real question here!

SAP is supposed to dephosphorylate nucleic acids, but I am not sure if it also dephosphorylates proteins, and if it does, in which aminoacids (Tyr, Thr, Ser).

I'm working with a Ras-related protein, which could be bound to GDP or GTP, SAP could be dephosphorylating these molecules, but as far as I understand, in a reducing western blot they should be released from the protein, am I right?

Thanks all!

P.D.: the results are from a PhosTag gel.