In many articles and chapters,primary concentrate was on cell impedance measurment but i can not find articles that focus on cell and ECM impedance measurment.
Hi,
which ECM do you mean?
Cell impedance can be measured with CellZscope from Nanoanalytics. It also determines trans endothelial resistance.
Hi
thanks for your conception Miss Kumar.
ECM is Abbreviated of extracellular Matrix.
My goals in my project is to measure impedance of ECM and scaffold in two situations, first The cells are not yet on the scaffold and second cells are on scaffold .
Do you have any idea for impedance measuring in these situations?
Hi,
I suggest you look into electric cell-substrate impedance sensing (ECIS). It's a non-invasive technique that gives sub-micro resolution, and real time recording of cell impedance.
Our company develops 3D cell barrier culture systems for in vitro study of virtually any cell barrier, and the system comes equipped with both a Fluid Perfusion Unit and a Trans-Endothelial/Epithelial Electrical Resistance (TEER) Measurement Unit! The use of a vessel-shaped 3D environment has been proven more effective than 2D systems like Transwell (
Article Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L...
), and our advanced TEER Measurement Unit allows for frequency sweeps between 0.1-1,000 Hz, automated time point sampling, logging of data to Excel, and additional measurement of phase angle for cell capacitance calculations! For those that are committed to Transwell use, we also have a TEER Measurement Unit that is compatible with nearly all Transwell products (Endohm cell cup chamber, STX2 "chopstick" electrodes, etc) and allows the user to greatly expand their testing capabilities with Transwell equipment. Additionally, we have smaller modular systems that connect to microscope-friendly cell culture modules: i.e., a miniaturized 3D cell culture system that you can use right under your microscope! If you or anybody on this thread is interested in learning more, I encourage you to visit www.flocel.com or email me at [email protected], thank you!
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