Dear researchers
I want to know polyubiquitin roles in DNA repair .(briefly)
Hi dear researchers Is it right use 1/mmol unit for extinction coefficient (molar absorption coefficient) instead of 1/mM? (I see this in some atticles)
11 December 2018 4,588 4 View
Dear researchers Why does DNA polymerase need an RNA primer? but RNA polymerase don't? What happened in catalytic site?
05 June 2018 5,034 0 View
Hi dear researchers I Want to know about metagenome and metataxonomic (definitions and applications). Why we need metagenomics? How metagenomics differ from genomics?
03 April 2018 3,457 0 View
Dear researchers, I want to know about torsion angles in nucleotide. Can someone help me?
31 December 2017 3,131 0 View
Dear researchers, Nanomaterials such as nanotubes and nanoporouses are used for immobilization of peroxidase enzymes. H2O2 is one of the Horseradish peroxidase enzyme substrates. Does oxidation...
31 December 2017 2,770 0 View
Dear researchers, I want to know about methods of producing recombinant insulin. Could anyone help me with some literature. Thanks in advance
11 December 2017 3,083 4 View
Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...
10 November 2017 5,244 3 View
Hi dear researchers According to this sentence "mRNA Spatial shape can effect on gene expression even the amount of gene expression" I waned to know how gene expression can be regulated by mRNA...
09 October 2017 3,344 4 View
I study a bout biosensors and bioreceptors... I am new biochemistry student...and I want to know a bout surface attachment of the biological element in biosensors... it is mystery for me ...
09 October 2017 1,374 4 View
Hi dears How do nanoparticles interact with enzymes after immobilization? How can bioinformatics tools be used for this? can I use bioinformatics free softwares with simple system to know...
01 January 1970 345 7 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View