I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the nucleotide binding pocket of DNA polymerase is too small to allow the presence of a 2'OH on the incoming nucleotide. But DNA polymerase require primer to initiate the replication. Primer is short RNA sequence of 5-10 bp. So how RNA polymerase is able to attach to the primer but not the ribonucleoside?
You've basically answered your own question. The DNA polymerase won't allow an RNA nucleotide as an INCOMING base. But it only needs a 3' -OH group to start polymerizing more DNA.
You've basically answered your own question. The DNA polymerase won't allow an RNA nucleotide as an INCOMING base. But it only needs a 3' -OH group to start polymerizing more DNA.
Dear Mansi,
Please follow this RG link
https://www.researchgate.net/post/Why_DNA_polymerase_can_not_start_DNA_synthesis_without_free_3-OH_but_RNA_polymerase_can_do_this
Watson and Crick first reasoned that complementary base pairing provides the basis of fidelity in DNA replication; that is, that each base in the template strand dictates the complementary base in the new strand. However, we now know that the process of DNA replication is very complex and requires the participation of many different components. Let’s examine each of these components and see how they fit together to produce our current picture of DNA synthesis in E. coli, the best-studied cellular replication system.
The Double Helix is wrong: there is no mutual twisting of the two strands -- they run in parallel. I proposed a different model, it is described in the article:
http://vixra.org/abs/1803.0104
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