Hello All,
I ordered some plasmids from Addgene that mention that they need to be propagated in Stbl3 E. coli. Addgene provides these in Stbl3 cells.
I want to generate competent Stbl3 cells for transformation. I am not finding a source for these in my institute and currently I do not want to order them. I was told previously that if plasmid carrying cells are streaked on LA without antibiotic, they lose the plasmid and can be used for competent cell preparation and re-transformation. Is this true? In that case can I plate my Addgene bacteria on LA and make them lose the plasmid?
Thanks in advance