What can I substitute for the Roche blocking reagent?

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Could we apply a strict carnivorous diet for laboratory mice or rats?

hello, a protocol requires a strict carnivorous diet for laboratory animals, can this diet be applied to mice and rats for 2 months (cheese, cooked chicken meat, eggs + vitamins in water)? thank you

06 January 2021 4,066 3 View

How to link Research Gate article citations to Google Scholar ?

Some publications which we upload in Research Gate receives citations but the same paper which has been listed in the Google Scholar doesn't show any citation .

01 January 2021 908 5 View

Calculation of dilution factor for fumonisin extracted rice cultured with fusarium species?

hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...

29 November 2020 1,831 15 View

Could the hepatic catalase activity increases when we treat mice with immunostimulants?

hello, I would like to know please, if there are cases in which we can observe an increase of catalase activity in mice treated with a therapeutic dose of immunostimulants? I did twice the...

28 September 2020 4,193 3 View

How to calculate the amount of toxin per gram of wheat?

hello, I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat? the...

17 March 2020 7,469 3 View

How I can get source code for 802.11a PHY layer in matlab without using simulink?

27 December 2019 2,191 3 View

Please someone tell me about IC50 on radical scavenging assay?

I've been measured the percent of my concentration series (R2 = 0.998) but the data weren't covered value more than 50%. Should I repeat my experiment or I just extrapolate the regression...

29 July 2019 2,899 5 View

What are the limitations for colorimetric in computer vision technique to extract object features with noisy background?

Combination of morphometric and colorimetric has been applied for feature extraction in detection algorithm. In noisy background, this CV technique facing wrong identification results as well as...

20 August 2018 845 6 View

How to validated the free radical inhibition on the following equation?

Dear All, I have find the different equation as usually to use for dpph inhibition on extract. How to validate the result that the equation was correct Please check the equation formula on the...

03 May 2018 1,394 5 View

What is the difference between Distributed based RAN (D-RAN) and Fog based RAN (F-RAN)?

In cloud-based RAN (C-RAN), all the signal processing were transferred from the local or small cell base stations (i.e. Distributed D-RAN) to a centralized center to simplify the network...

05 February 2018 2,941 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Najib Abdellaoui

Laurence Stuart Dawkins-Hall

thank you for your answer. for the hybridization buffer, i am using the thermofisher hybridization buffer. so i think it is possible to use another one. which one will be more suitable for the southern blot?

nope, i meant the hybridization buffer for the probe, because i am using the thermofisher hybridization buffer. so i was thinking to use another hybridization buffer instead of thermofisher's one.

thank you for your time and help. I will try the different hybridization buffer.