I'm a cheap-skate and don't want to keep buying the NEB stable shots. I regularly make my own XL-10, DH5 and BL25 (Hanahan protocol) competent cells, but am wondering if anybody has any experience with doing the same with the NEB Stable lines?
I get that I'll have to grow them at the lower temp, but I was wondering if anybody else had any nuggets of wisdom before I attempt.
Thanks!
Dan