I plan to use Lipofectamin 2000 to transfect human macrophage in vitro. Macrophage is not a easy target for tranfection and there are not many papers on tihs topic. Does someone has some experience to share? Thanks in advance!
Avoid using Lipofectamine and other lipid-based transfection reagents with macrophages because the lipids themselves will be too toxic. Better to use non-lipid cationic complexes like FuGENE or jetPEI-Macrophage. Also consider Nucleofection technologies.
Additional note: The above is true for most macrophages, however RAW264.7 is more likely to be an easy transfection target because they are deficient in ASC and have a muted response to the lipotoxicity of lipid-based reagents. However, they are non-functional for some signaling pathways, such as inflammasome signaling.
Hi! If you do not know them already, I would suggest you to try the Viromers. They will give you more than 80% of knock-down efficiency for siRNA transfection. If you transfect plasmid in RAW macrophages, Viromer RED will work, but for primary macrophages plasmid transfection is limited due to their self-defense mechanisms (AIM2/IL and cGAS/IF enzymatic cacscades induced by foreign DNA). Transfecting mRNA instead (if possible modified mRNA) is the best alternative... Do not hesitate to contact me if you want test samples. Bests, Sandra
https://viromer-transfection.com/macrophage-transfection
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques