I am looking for rough recommended concentrations but I have only found in vitro treatment concentrations.
Are you sure that these GTP analogues would cross the plasma membrane and enter your cells?
Apart from that in my experience the Kd of many enzymes for AMP-PNP is about
10, that for AMP-PCP about 100 times that for ATP (e.g., Biochem. J. 318
(1996) 923-929), if things are similar with GTP then you should reach 10-100
times the cytosolic concentration of GTP (500 µM) to see an effect.
@ARTICLE{Tra-94,
AUTHOR = {T.W. Traut},
TITLE = {Physiological concentrations of purines and pyrimidines},
YEAR = {1994},
JOURNAL = {Molecular and Cellular Biochemistry},
volume = {140},
url = {http://rasmodel.org/_downloads/7877593.pdf},
pages = {1-22},
}
This might constitute a logistic problem, as such nucleotid analogues are very expensive. You should also check them for contammination with GDP and GMP, which could give you misleading results.
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