I am trying to prepare agar plates from the CHROMagar powder and the instructions say to mix 33g in 1L of water and bring to the boil at 100 degrees before autoclaving. This is difficult for me as my lab does not have bunsen burners or waterbaths with this capability. I was wondering has anybody tried making this agar without boiling the solution before autoclaving. Thanks
Do it on a microwave oven.
Also, if your autoclave is an old model (where you can remove items from the sterilization chamber while the temperature is still relatively high) you might try autoclaving directly, without melting the agar beforehand, and then homogenizing the resulting solution afterwards.
When prepare MS/agar medium for plants, I saw some protocols suggesting to melt the agar first, then autoclave. I have never done that. I autoclave them directly (as Alejandro mentioned). For that, I did not see any obvious detrimental effect on tissue-cultured plants. I don't use CHROM agar, but you can try out by directly autoclaving them.
If you are following instructions that say ;boil the media to mix agar completely & then autoclave it'- then you can directly autoclave the media then mix the molten agar completely to get uniform solution by swirling & pour the plates. It will not hamper the agar properties.
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