We are currently using the digestion solution below to digest cells and matrix grown within 2% agarose gels however large pieces of agarose still remain. Any suggestions on how to trouble-shoot or does anyone have any alternative protocols for digesting agarose gels?

125 μg/ml papain (Sigma P 4762) 

0.1 M sodium phosphate (NaH2PO4.H20) 

5 mM EDTA (C10H16N2O8) 

5 mM cysteine hydrochloride

pH 6.5

60 degreesC, overnight digestion with agitation

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