RNA profile for all my samples shows strange big peak at 5S position. Should I use these RNA samples for downstream experiments? Does anyone know if this is common with Staphylococcus aureus. Total RNA was isolated by using RNA protect (Qiagen) for stabilisation of RNA. RNeasy kit was used for extraction. I have also noticed that the concentration of RNA is differs with the instruments used. Nanodrop gives higher concentration than Bioanalyser for the same samples. Please let me know what you think regarding the peak shown in attached image. I have very limited experience with RNA and seeking for experts help. I am happy to provide any other detail you might want to know. Thank you very much.

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