Dear all, while SB431542 is established to block TGFbeta and LDN193189 to inhibit the BMP pathway, I was wondering if anyone has seen the combined effect of the two in neural differentiation of mouse embryonic stem cells? Their combined effect causes neuron production in human ESCs but I couldn't find any literature for mESCs. (http://www.nature.com/nbt/journal/v35/n2/full/nbt.3777.html)
Is SB431542 only used to maintain pluripotency and inhibit differentiation in the mESCs instead ? (https://www.ncbi.nlm.nih.gov/pubmed/24949833)
Would appreciate your experiences/ suggestions/ ideas.
Many thanks.
Pooja.
Both GFP positive and GFP negative cell population decrese phosphorylation when you use LDN and SB together. I read this paper some time ago. Please see this paper and you may write to Author if you need. here is that " TGF-beta-superfamily signaling regulates embryonic stem cell heterogeneity: self-renewal as a dynamic and regulated equilibrium" published in stem cells January 2013.
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